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NATURE新闻论韩氏NgAgo以及我的评论

热度 1已有 4033 次阅读2016-8-9 08:41 |个人分类:科普|系统分类:时事

《自然》杂志网站登出了新闻,标题是《Replications, ridicule and a recluse: the controversy over NgAgo gene-editing intensifies》(重复、嘲讽与隐士:NgAgo 基因编辑风波越演越烈)。链接如下,http://www.nature.com/news/replications-ridicule-and-a-recluse-the-controversy-over-ngago-gene-editing-intensifies-1.20387

文中报道了方舟子的作假指控,澳洲 BURGIO博士的失败,以及某不愿透露姓名的中国学者的部分成功(包括基因测序验证)(One researcher in China, who works independently from Han’s research group and who doesn’t want his name to be entangled in the public controversy, told Nature that he had tested NgAgo in a few kinds of cell and found that it was able to induce genetic mutations at the desired sites — a finding that he verified by sequencing.)。

我在 NATURE 文后的评论总结:我在上面的评论如下(英译中):

下面是我对中国发生的有关韩氏 NgAgo 激烈辩论的一个总结。

1. 方舟子鲁莽的作假指控仅仅是由于他未能理解韩的论文及分子生物概念不清。方舟子的一个超级粉丝都向他指出他对论文中电泳图的解读错误。方还把基因编辑靶点的距离当成了DNA片段的大小差。

2. 另外一个实名人士(方的主要信息来源)在网上的直接辩论中丧失可信度,因为未考虑 NgAgo 的剪切机制(在靶点附近去掉多达20个核苷酸)。

3. 正如韩的论文所说, NgAgo 蛋白只有在表达时才能装载导引。澳洲BURGIO博士承认他没有按照韩论文中重点强调的程序操作(那又怎么能成功?)

至此,所谓作假的指控已被证明为缺乏根据。一次成功的重复就足以定性地证明韩的结果。但愿对韩的怀疑与嘲讽将引发更多的好奇与研究并得出更为确定的答案,而不是因为误导而放弃一个可能具有巨大潜力的基因编辑工具。

The following is a summary of some very heated debate in China on the Han NgAgo paper.

1. Fang's rash accusation of fraud was a mere result of his misunderstandings of the Han paper and molecular biology in general. Even one of Fang's ardent supporters pointed out to him that he misinterpreted the electrophoresis bands presented in the paper. Fang further mistook distances between target sites for DNA segment size deltas.

2. Another named person, who was Fang's main source, has been discredited in a direct online debate, for failing to consider NgAgo's effect of removing 1 to 20 nts at the target site.

3. As stated in Han's paper, the guide can be loaded only when the NgAgo protein is in the process of expression. Dr. Burgio admitted that he did not follow a starred procedure in Han's published protocol.

Thus far, the accusations of fraud have been shown to be unfounded. A single successful replication is sufficient to qualitatively vindicate Han's result. Hopefully, the suspicion and ridicule will trigger more curiosity and research to bring about more definitive answers, instead of misguided abandonment of a potentially potent tool for gene editing.


我的相关博文链接:
专家:方舟子一窍不通
 澳洲 BURGIO 教授未按韩氏流程实验

NgAgo 研究者发表注意事项


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发表评论 评论 (1 个评论)

回复 稻草 2016-8-9 09:06
这完全是中国人包括一些海外华人的自卑心导致的质疑。在它们看来,如果是欧美的人搞的实验,错的也是对的。

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